验证数据展示
经过测试的应用
| Positive FC detected in | mouse peritoneal macrophages |
For other applications, we recommend the unconjugated version of this antibody, 65221-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65221 targets B7-H3/CD276 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Mouse IgG2a Fc-CD276 (aa 1-242) fusion protein 种属同源性预测 |
| 宿主/亚型 | Rat / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD276 antigen |
| 别名 | CD276, CD276 antigen, B7RP 2, B7-H3, B7h3 |
| GenBank蛋白编号 | BC019436 |
| 基因名称 | Cd276 |
| Gene ID (NCBI) | 102657 |
| RRID | AB_3084170 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8VE98 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD276 (also known as B7-H3) is a type I transmembrane protein expressed on many tissues and cell types. CD276 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, CD276 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 B7-H3/CD276 antibody CL488-65221 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |