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验证数据展示

  • FC experiment of human PBMCs using AY7-FcA98346-3

    FC experiment of human PBMCs using AY7-FcA98346-3

    1x10^6 human PBMCs were surface stained with FcZero-rAb™ CoraLite® Plus 488 Anti-Human CD3 (UCHT1), and 5 ul APC-Cyanine7 Anti-Human CD27 Rabbit RecAb (AY7-FcA98346-3, Clone: 242731G8) or FcZero-rAb™ APC-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (AY7-FcA98136, Clone: 240953C9). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using AY7-FcA98346-3

    FC experiment of human PBMCs using AY7-FcA98346-3

    1x10^6 human PBMCs were surface stained with 5 ul APC-Cyanine7 Anti-Human CD27 Rabbit RecAb (AY7-FcA98346-3, Clone: 242731G8) (red) or FcZero-rAb™ APC-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (AY7-FcA98136, Clone: 240953C9) (blue). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were not fixed. Lymphocytes were gated.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® APC-Cyanine7 Anti-Human CD27 Rabbit Recombinant Antibody

CD27 FcZero-rAb® Recombinant Antibody for FC
Cat No. AY7-FcA98346-3

产品说明书

CloneNo. 242731G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  APC-Cyanine7
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

AY7-FcA98346-3 targets CD27 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1644

Product name: Recombinant Human CD27 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 20-191 aa of BC012160

Sequence: ATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD27 molecule
别名 CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27
计算分子量 29 kDa
GenBank蛋白编号BC012160
基因名称 CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
RRIDAB_3746713
偶联类型 APC-Cyanine7 Fluorescent Dye
最大激发/发射波长650 nm / 778 nm
激发激光Red Laser (633 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26842
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

实验方案

Product Specific Protocols
FC protocol for APC-Cyanine7 CD27 antibody AY7-FcA98346-3Download protocol
Standard Protocols
Click here to view our Standard Protocols
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