×
Use Able AI chat for product recommendations

验证数据展示

  • FC experiment of human PBMCs using PE-FcA98469-4

    FC experiment of human PBMCs using PE-FcA98469-4

    1x10^6 PHA-treated or untreated human PBMCs were surface stained with FcZero-rAb® APC Anti-Human CD3 and 5 ul PE Anti-Human CD25/IL-2RA Rabbit RecAb (PE-FcA98469-4, Clone: 253320A1). Cells were not fixed.

  • FC experiment of human PBMCs using PE-FcA98469-4

    FC experiment of human PBMCs using PE-FcA98469-4

    1x10^6 PHA-treated human PBMCs were surface stained with 5 ul PE Anti-Human CD25/IL-2RA Rabbit RecAb (PE-FcA98469-4, Clone: 253320A1) (red) or FcZero-rAb® PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human CD25/IL-2RA Rabbit RecAb (PE-FcA98469-4, Clone: 253320A1) (black, dashed). Cells were not fixed.

  • FC experiment of Cnyo PBMCs using PE-FcA98469-4

    FC experiment of Cnyo PBMCs using PE-FcA98469-4

    1x10^6 cynomolgus PBMCs were surface stained with FcZero-rAb® APC Anti-Human CD3, and 5 ul PE Anti-Human CD25/IL-2RA Rabbit RecAb (PE-FcA98469-4, Clone: 253320A1) or FcZero-rAb® PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® PE Anti-Human CD25/IL-2RA Rabbit Recombinant Antibody

CD25/IL-2RA FcZero-rAb® Recombinant Antibody for FC
Cat No. PE-FcA98469-4

产品说明书

CloneNo. 253320A1

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey, non-human primates

应用

FC

别名

CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  PE
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inPHA treated human PBMCs, Cynomolgus PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-FcA98469-4 targets CD25/IL-2RA in FC applications and shows reactivity with human, monkey, non-human primates samples.

经测试应用 FC Application Description
经测试反应性 human, monkey, non-human primates
免疫原

unknow

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 2 receptor, alpha
别名 CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA
基因名称 IL2RA
Gene ID (NCBI) 3559
RRIDAB_3749900
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
激发激光Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for PE CD25/IL-2RA antibody PE-FcA98469-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...