验证数据展示
经过测试的应用
| Positive FC detected in | anti-CD3/CD28 treated mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CPY5-FcA98288 targets CD25/IL-2RA in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg3087 Product name: Recombinant Mouse CD25/IL2RA protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 22-236 aa of NM_008367.3 Sequence: ELCLYDPPEVPNATFKALSYKNGTILNCECKRGFRRLKELVYMRCLGNSWSSNCQCTSNSHDKSRKQVTAQLEHQKEQQTTTDMQKPTQSMHQENLTGHCREPPPWKHEDSKRIYHFVEGQSVHYECIPGYKALQRGPAISICKMKCGKTGWTQPQLTCVDEREHHRFLASEESQGSRNSSPESETSCPITTTDFPQPTETTAMTETFVLTMEYK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 2 receptor, alpha chain |
| 别名 | CD25, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha, Il2ra |
| 计算分子量 | 31 kDa |
| GenBank蛋白编号 | NM_008367.3 |
| 基因名称 | CD25 |
| Gene ID (NCBI) | 16184 |
| 偶联类型 | PerCP-Cyanine5.5 Fluorescent Dye |
| 最大激发/发射波长 | 482 nm / 690nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01590 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PerCP-Cyanine5.5 CD25/IL-2RA antibody CPY5-FcA98288 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
