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Conjugate
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验证数据展示

  • FC experiment of mouse splenocytes using CL647-98288

    FC experiment of mouse splenocytes using CL647-98288

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were stained with CoraLite® Plus 488 Anti-Mouse CD4 and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD25/IL-2RA Rabbit RecAb (CL647-98288, Clone: 242494H5). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL647-98288

    FC experiment of mouse splenocytes using CL647-98288

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD25/IL-2RA Rabbit RecAb (CL647-98288, Clone: 242494H5) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD25/IL-2RA Rabbit RecAb (CL647-98288, Clone: 242494H5) (black, dashed). Cells were not fixed.

CoraLite® Plus 647 Anti-Mouse CD25/IL-2RA Rabbit Recombinant Antibody

CD25/IL-2RA Uni-rAbTM Recombinant Antibody for FC
Cat No. CL647-98288

产品说明书

CloneNo. 242494H5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD25, 242494H5, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC detected inAnti-CD3/CD28 treated mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 98288-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98288 targets CD25/IL-2RA in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 CD25/IL-2RA fusion protein Eg3087 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 2 receptor, alpha chain
别名 CD25, 242494H5, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha
计算分子量31 kDa
GenBank蛋白编号NM_008367.3
基因名称 CD25
Gene ID (NCBI) 16184
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01590
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD25/IL-2RA antibody CL647-98288Download protocol
Standard Protocols
Click here to view our Standard Protocols