验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 98176-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-98176 targets CD24 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2104 Product name: Recombinant Mouse CD24 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 27-53 aa of NM_009846.2 Sequence: NQTSVAPFPGNQNISASPNPSNATTRG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD24a antigen |
| 别名 | 241691D7, Cd24a, CD24a antigen, HSA, Ly 52 |
| 计算分子量 | 8 kDa |
| GenBank蛋白编号 | NM_009846.2 |
| 基因名称 | CD24 |
| Gene ID (NCBI) | 12484 |
| RRID | AB_3747155 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 激发激光 | Violet Laser (405 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P24807 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CD24 antibody CL405-98176 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
