• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD22 Monoclonal antibody

CD22 Monoclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

7A2F1

Cat No : 66103-1-Ig

Print datasheet

Synonyms

B cell receptor CD22, BL CAM, CD22, CD22 molecule, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14

验证数据展示

  • WB analysis of Ramos using 66103-1-Ig

    WB analysis of Ramos using 66103-1-Ig

    Ramos, Daudi, and Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 66103-1-Ig

    WB analysis of Raji using 66103-1-Ig

    Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • IHC staining of human tonsillitis using 66103-1-Ig

    IHC staining of human tonsillitis using 66103-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD22 antibody included
  • IHC staining of human tonsillitis using 66103-1-Ig

    IHC staining of human tonsillitis using 66103-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD22 antibody included
  • IF Staining of human tonsillitis using 66103-1-Ig

    IF Staining of human tonsillitis using 66103-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 66103-1-Ig

    IF Staining of human tonsillitis using 66103-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inRamos cells, Raji cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, Raji cells
Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66103-1-Ig targets CD22 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen CD22 fusion protein Ag17986 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD22 molecule
Synonyms B cell receptor CD22, BL CAM, CD22, CD22 molecule, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14
Calculated Molecular Weight 847 aa, 95 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC109306
Gene Symbol CD22
Gene ID (NCBI) 933
RRIDAB_2881502
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

实验方案

Product Specific Protocols
WB protocol for CD22 antibody 66103-1-IgDownload protocol
IHC protocol for CD22 antibody 66103-1-IgDownload protocol
IF protocol for CD22 antibody 66103-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Biomed Res

Causal genetic regulation of DNA replication on immune microenvironment in colorectal tumorigenesis: evidenced by an integrated approach of trans-omics and GWAS

Authors - Sumeng Wang
View Article