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验证数据展示

  • WB analysis using 32183-1-AP

    WB analysis using 32183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32183-1-AP (CD22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse spleen using 32183-1-AP

    IF Staining of mouse spleen using 32183-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD22 antibody (32183-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of mouse splenocytes using 32183-1-AP

    FC experiment of mouse splenocytes using 32183-1-AP

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CD22 Polyclonal antibody (32183-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug (CL647-65290), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

CD22 Polyclonal antibody

CD22 Polyclonal Antibody for WB, IF-P, FC, ELISA
Cat No. 32183-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF-P, FC, ELISA

别名

Siglec2, Sialic acid-binding Ig-like lectin 2, Lyb-8, B-lymphocyte cell adhesion molecule, BL-CAM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IF-P detected inmouse spleen tissue
Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32183-1-AP targets CD22 in WB, IF-P, FC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF-P, FC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD22 antigen
别名 Siglec2, Sialic acid-binding Ig-like lectin 2, Lyb-8, B-lymphocyte cell adhesion molecule, BL-CAM
计算分子量97kDa
观测分子量 120-150 kDa
GenBank蛋白编号NM_001043317.2
基因名称 Cd22
Gene ID (NCBI) 12483
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP35329-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

实验方案

Product Specific Protocols
WB protocol for CD22 antibody 32183-1-APDownload protocol
IF protocol for CD22 antibody 32183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols