• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using FITC-65198

    FC experiment of human PBMCs using FITC-65198

    1X10^6 human PBMCs were surface co-stained with CL647-CD19 and 5 ul FITC Plus Anti-Human CD21 (FITC-65198, Clone: BU32), or FITC Plus Mouse IgG1 Isotype Control (FITC-65125, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using FITC-65198

    FC experiment of human PBMCs using FITC-65198

    1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD21 (FITC-65198, Clone: BU32), or FITC Plus Mouse IgG1 Isotype Control (FITC-65125, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

FITC Plus Anti-Human CD21 (BU32)

CD21 Monoclonal Antibody for FC
Cat No. FITC-65198

产品说明书

CloneNo. BU32

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

BU32, C3DR, Complement C3d receptor, Complement receptor type 2, CR2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  FITC Plus
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65198 targets CD21 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component (3d/Epstein Barr virus) receptor 2
别名 BU32, C3DR, Complement C3d receptor, Complement receptor type 2, CR2
计算分子量 1092 aa, 119 kDa
GenBank蛋白编号BC136394
基因名称 CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
RRIDAB_2924129
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20023
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD21 antibody FITC-65198Download protocol
Standard Protocols
Click here to view our Standard Protocols