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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 98091-2-RR

    FC experiment of human PBMCs using 98091-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit RecAb (98091-2-RR, Clone: 241329G4) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98091-2-RR

    FC experiment of human PBMCs using 98091-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit RecAb (98091-2-RR, Clone: 241329G4) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98091-2-RR

    Affinity and Kinetic Characterization of 98091-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98091-2-RR against Human CD200 were performed. The affinity constant is 0.405 nM.

Anti-Human CD200 Rabbit Recombinant Antibody

CD200 Recombinant Antibody for FC
Cat No. 98091-2-RR

产品说明书

CloneNo. 241329G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

241329G4, CD200 molecule, MOX1, MOX2, MRC

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98091-2-RR targets CD200 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD200 molecule
别名 241329G4, CD200 molecule, MOX1, MOX2, MRC
计算分子量 269 aa, 30 kDa
GenBank蛋白编号BC022522
基因名称 CD200
Gene ID (NCBI) 4345
RRIDAB_3672238
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP41217
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

实验方案

Product Specific Protocols
FC protocol for CD200 antibody 98091-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols