验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Positive IF/ICC detected in | Raji cells |
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86480-1-RR targets CD20 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | membrane-spanning 4-domains, subfamily A, member 1 |
| 别名 | B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35 |
| 计算分子量 | 297 aa, 33 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC002807 |
| 基因名称 | CD20 |
| Gene ID (NCBI) | 931 |
| RRID | AB_3744914 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11836-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CD20 antibody 86480-1-RR | Download protocol |
| WB protocol for CD20 antibody 86480-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
