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验证数据展示

  • WB analysis using 86480-1-RR (same clone as 86480-1-PBS)

    WB analysis using 86480-1-RR (same clone as 86480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86480-1-RR (MS4A1,CD20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86480-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 86480-1-RR (same clone as 86480-1-PBS)

    IF Staining of Jurkat using 86480-1-RR (same clone as 86480-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji / Jurkat cells using CD20 antibody (86480-1-RR, Clone: 251207B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86480-1-PBS in a different storage buffer formulation.

CD20 Recombinant monoclonal antibody, PBS Only

CD20 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86480-1-PBS

产品说明书

CloneNo. 251207B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.

产品信息

86480-1-PBS targets CD20 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 membrane-spanning 4-domains, subfamily A, member 1
别名 B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35
计算分子量 297 aa, 33 kDa
观测分子量35 kDa
GenBank蛋白编号BC002807
基因名称 CD20
Gene ID (NCBI) 931
RRIDAB_3744914
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11836-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

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