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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)

    FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65565-1-PBS

    FC experiment of human PBMCs using 65565-1-PBS

    1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) , or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with FITC Plus Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only

CD2 Uni-rAb® Recombinant Antibody for FC
Cat No. 65565-1-PBS

产品说明书

CloneNo. TS1/8

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65565-1-PBS targets CD2 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

N/A

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD2 molecule
别名 CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor
计算分子量 351 aa, 39 kDa
GenBank蛋白编号BC033583
基因名称 CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06729
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
FC protocol for CD2 antibody 65565-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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