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验证数据展示

  • IHC staining of human tonsillitis using 28838-1-AP

    IHC staining of human tonsillitis using 28838-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28838-1-AP (CD1c antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 28838-1-AP

    IHC staining of human tonsillitis using 28838-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28838-1-AP (CD1C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD1c Polyclonal antibody

CD1c Polyclonal Antibody for IHC, ELISA
Cat No. 28838-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

BDCA1, CD1, CD1c molecule, R7, T-cell surface glycoprotein CD1c

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28838-1-AP targets CD1c in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29830

Product name: Recombinant human CD1C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 18-118 aa of BC126467

Sequence: NADASQEHVSFHVIQIFSFVNQSWARGQGSGWLDELQTHGWDSESGTIIFLHNWSKGNFSNEELSDLELLFRFYLFGLTREIQDHASQDYSKYPFEVQVKA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD1c molecule
别名 BDCA1, CD1, CD1c molecule, R7, T-cell surface glycoprotein CD1c
计算分子量 333 aa, 38 kDa
GenBank蛋白编号BC126467
基因名称 CD1C
Gene ID (NCBI) 911
RRIDAB_3086090
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29017
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD1c molecule (CD1c, also known as R7, CD1, CD1A, and BDCA1) is a member of the CD1 family of transmembrane glycoproteins, which is synthesized in the endoplasmic reticulum and expressed on the monocytes, marginal zone B cells in lymph nodes as well as peripheral blood (PMID: 23468110). The CD1 proteins belong to the family of major histocompatibility complex (MHC) class I-like proteins that present lipid-based antigens to T cells (PMID: 15032598; 23127489). CD1c shows interaction with both αβ or γδ T cells and mediates T cell responses against mycobacterium tuberculosis (PMID: 10727456; 10786796).

实验方案

Product Specific Protocols
IHC protocol for CD1c antibody 28838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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