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Anti-Mouse CD197 (4B12)

CD197 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

4B12

Cat No : 65158-1-Ig

Print datasheet

Synonyms

Ccr7, CD197, Cdw197, Cmkbr7, EBI1, Ebi1h

验证数据展示

  • FC experiment of mouse splenocytes using 65158-1-Ig

    FC experiment of mouse splenocytes using 65158-1-Ig

    1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 1 ug Anti-Mouse CD197 (65158-1-Ig, Clone: 4B12) and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

  • FC experiment of mouse splenocytes using 65158-1-Ig

    FC experiment of mouse splenocytes using 65158-1-Ig

    1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 1 ug Rat IgG2a Isotype Control and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65158-1-Ig targets CD197 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen Mouse CCR7 Transfected Cell Line 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name chemokine (C-C motif) receptor 7
Synonyms Ccr7, CD197, Cdw197, Cmkbr7, EBI1, Ebi1h
Gene Symbol Ccr7
Gene ID (NCBI) 12775
RRIDAB_2918440
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-G affinity chromatography
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for CD197 antibody 65158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols