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CD163 Monoclonal antibody

CD163 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G7A5

Cat No : 68218-1-Ig

Print datasheet

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

验证数据展示

  • WB analysis of human placenta using 68218-1-Ig

    WB analysis of human placenta using 68218-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68218-1-Ig (CD163 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 68218-1-Ig

    IHC staining of human liver using 68218-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 68218-1-Ig

    IHC staining of human liver using 68218-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver using 68218-1-Ig

    IF Staining of human liver using 68218-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver using 68218-1-Ig

    IF Staining of human liver using 68218-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman liver tissue, human placenta tissue, human tonsillitis tissue, human lung cancer tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68218-1-Ig targets CD163 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human
Immunogen CD163 fusion protein Ag26721 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD163 molecule
Synonyms CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163
Calculated Molecular Weight 1156 aa, 125 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86VB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

实验方案

Product Specific Protocols
WB protocol for CD163 antibody 68218-1-IgDownload protocol
IHC protocol for CD163 antibody 68218-1-IgDownload protocol
IF protocol for CD163 antibody 68218-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols