验证数据展示
65057-1-Ig
产品信息
65057-1-PBS targets CD16/CD32 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 | VH81X Tg B cells and T220 stromal cell line 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2a, lambda |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Fc receptor, IgG, low affinity IIb |
| 别名 | CD32, 93, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB |
| GenBank蛋白编号 | BC038070 |
| 基因名称 | CD16/32 |
| Gene ID (NCBI) | 14130 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P08101 |
| 储存缓冲液 | PBS Only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.