CoraLite® Plus 488 Anti-Mouse CD154 (MR1)
CD154 Monoclonal Antibody for FC
Host / Isotype
Armenian Hamster / IgG
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
MR1
验证数据展示
经过测试的应用
| Positive FC detected in | Cell Stimulation Cocktail stimulated mouse CD4+ T cells |
For other applications, we recommend the unconjugated version of this antibody, 65129-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65129 targets CD154 in FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC |
| Tested Reactivity | Mouse |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD40 ligand |
| Synonyms | CD154, CD40 ligand, Cd40l, Cd40lg, gp39, HIGM1, IGM, IMD3, Ly 62, Ly62, MR1, T BAM, Tnfsf5, TRAP |
| GenBank Accession Number | BC119225 |
| Gene Symbol | Cd40lg |
| Gene ID (NCBI) | 21947 |
| RRID | AB_2919266 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD154 antibody CL488-65129 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
