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验证数据展示

  • FC experiment of human PBMCs using 98558-1-RR

    FC experiment of human PBMCs using 98558-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD147 Rabbit RecAb (98558-1-RR, Clone: 251215H5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98558-1-RR

    FC experiment of human PBMCs using 98558-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD147 Rabbit RecAb (98558-1-RR, Clone:251215H5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98558-1-RR

    Affinity and Kinetic Characterization of 98558-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98558-1-RR against Human CD147 were performed. The affinity constant is 1.08 nM.

Anti-Human CD147 Rabbit Recombinant Antibody

CD147 Uni-rAb® Recombinant Antibody for FC
Cat No. 98558-1-RR

产品说明书

CloneNo. 251215H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

5F7, Basigin, BSG, Collagenase stimulatory factor, EMMPRIN

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD147/BSG Ready-To-Use IHC Kit. CD147/BSG primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98558-1-RR targets CD147 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg0436

Product name: Recombinant Human CD147 protein (His Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 138-323 aa of BC009040

Sequence: EPGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 basigin (Ok blood group)
别名 5F7, Basigin, BSG, Collagenase stimulatory factor, EMMPRIN
计算分子量 385 aa, 42 kDa
GenBank蛋白编号BC009040
基因名称 CD147
Gene ID (NCBI) 682
RRIDAB_3746178
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35613
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211).

实验方案

Product Specific Protocols
FC protocol for CD147 antibody 98558-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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