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验证数据展示

  • IF Staining of HUVEC using CL488-17564

    IF Staining of HUVEC using CL488-17564

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 CD146/MCAM antibody (CL488-17564) at dilution of 1:400.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CD146/MCAM Polyclonal antibody

CD146/MCAM Polyclonal Antibody for IF/ICC
Cat No. CL488-17564

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

CD146, S-endo 1 endothelial-associated antigen, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD146/MCAM Ready-To-Use IHC Kit. CD146/MCAM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17564-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17564 targets CD146/MCAM in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 CD146/MCAM fusion protein Ag11762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma cell adhesion molecule
别名 CD146, S-endo 1 endothelial-associated antigen, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32
计算分子量 646 aa, 72 kDa
观测分子量 113-120 kDa
GenBank蛋白编号BC056418
基因名称 CD146
Gene ID (NCBI) 4162
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43121
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD146/MCAM antibody CL488-17564Download protocol
Standard Protocols
Click here to view our Standard Protocols