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验证数据展示

  • FC experiment of A375 using CL555-65181

    FC experiment of A375 using CL555-65181

    1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD146 (CL555-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

  • FC experiment of A375 using CL555-65181

    FC experiment of A375 using CL555-65181

    1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD146 (CL555-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

CoraLite® Plus 555 Anti-Human CD146/MCAM (P1H12)

CD146/MCAM Monoclonal Antibody for FC
Cat No. CL555-65181

产品说明书

CloneNo. P1H12

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human, mouse, rabbit, canine

应用

FC

别名

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

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经过测试的应用

Positive FC detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy CD146/MCAM Ready-To-Use IHC Kit. CD146/MCAM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65181-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65181 targets CD146/MCAM in FC applications and shows reactivity with human, mouse, rabbit, canine samples.

经测试应用 FC Application Description
经测试反应性 human, mouse, rabbit, canine
免疫原 cultured human umbilical cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 melanoma cell adhesion molecule
别名 CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18
计算分子量 646 aa, 72 kDa
GenBank蛋白编号BC056418
基因名称 CD146
Gene ID (NCBI) 4162
RRIDAB_3084580
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP43121
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD146/MCAM antibody CL555-65181Download protocol
Standard Protocols
Click here to view our Standard Protocols