验证数据展示
经过测试的应用
| Positive FC detected in | U266 cells |
Planning an IHC experiment? We recommend our IHCeasy CD138/Syndecan-1 Ready-To-Use IHC Kit. CD138/Syndecan-1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98051-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-98051 targets CD138/Syndecan-1 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 | CD138/Syndecan-1 fusion protein Eg0132 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | syndecan 1 |
| 别名 | CD138, 240422C4, SDC, SDC1, SYND1 |
| 计算分子量 | 32.5 kDa |
| GenBank蛋白编号 | BC008765 |
| 基因名称 | CD138 |
| Gene ID (NCBI) | 6382 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P18827 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 CD138/Syndecan-1 antibody CL594-98051 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |