验证数据展示
经过测试的应用
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | mouse bone marrow cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
| FC | See 1 publications below |
产品信息
CL488-65055 targets CD11b in IF/ICC, FC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, FC Application Description |
| 文献引用应用 | IF, FC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin alpha M |
| 别名 | CR3, CR3A, integrin alpha M, Itgam, Ly 40 |
| 计算分子量 | 127 kDa |
| GenBank蛋白编号 | BC156991 |
| 基因名称 | CD11b |
| Gene ID (NCBI) | 16409 |
| RRID | AB_2883153 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | E9Q604 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD11b antibody CL488-65055 | Download protocol |
| FC protocol for CL Plus 488 CD11b antibody CL488-65055 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Vet Res Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis | ||
Biomed Pharmacother Mitochondrion-NLRP3 inflammasome activation in macrophages: A novel mechanism of the anti-inflammatory effect of Notopterygium in rheumatoid arthritis treatment |