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验证数据展示

  • IF Staining of human tonsillitis using CL594-66256

    IF Staining of human tonsillitis using CL594-66256

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11a/Integrin Alpha L antibody (CL594-66256, Clone: 1E3H12 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-66256

    IF Staining of human tonsillitis using CL594-66256

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11a/Integrin Alpha L antibody (CL594-66256, Clone: 1E3H12 ) at dilution of 1:200.

CoraLite®594-conjugated CD11a/Integrin Alpha L Monoclonal antibody

CD11a/Integrin Alpha L Monoclonal Antibody for IF-P
Cat No. CL594-66256

产品说明书

CloneNo. 1E3H12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF-P

别名

CD11A, CD11a/Integrin Alpha L, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 66256-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66256 targets CD11a/Integrin Alpha L in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原

CatNo: Ag7813

Product name: Recombinant human CD11a/Integrin alpha-L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-350 aa of BC008777

Sequence: SYNLDVRGARSFSPPRAGRHFGYRVLQVGNGVIVGAPGEGNSTGSLYQCQSGTGHCLPVTLRGSNYTSKYLGMTLATDPTDGSILFAAVQFSTSYKTEFDFSDYVKRKDPDALLKHVKHMLLLTNTFGAINYVATEVFREELGARPDATKVLIIITDGEATDSGNIDAAKDIIRYIIGIGKHFQTKESQETLHKFASKPASEFVKILDTFEKLKDLFTELQKKIYVIEGTSKQDLTSFNMELSSSGISADLSRGHAVVGAVGAKDWAGGFLDLKADLQDDTFIGNEPLTPEVRAGYLGYTVTWLPSRQKTSLLASGAPRYQHMGRV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
别名 CD11A, CD11a/Integrin Alpha L, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A
计算分子量 129 kDa
GenBank蛋白编号BC008777
基因名称 CD11a
Gene ID (NCBI) 3683
ENSEMBL Gene IDENSG00000005844
RRIDAB_2919972
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP20701
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

实验方案

Product Specific Protocols
IF protocol for CL594 CD11a/Integrin Alpha L antibody CL594-66256Download protocol
Standard Protocols
Click here to view our Standard Protocols