验证数据展示
经过测试的应用
| Positive FC detected in | mouse thymocytes |
For other applications, we recommend the unconjugated version of this antibody, 65177-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-65177 targets CD11a in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | Mouse |
| 免疫原 | C57BL/6 mouse splenic secondary cytotoxic T cells 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin alpha L |
| 别名 | Cd11a, integrin alpha L, Itgal, LFA 1, Ly 15, Ly 21 |
| GenBank蛋白编号 | BC145802 |
| 基因名称 | CD11a |
| Gene ID (NCBI) | 16408 |
| RRID | AB_3084578 |
| 偶联类型 | CoraLite® Plus 555 Fluorescent Dye |
| 最大激发/发射波长 | 554 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Purified by protein-A affinity chromatography |
| UNIPROT ID | B7ZN92 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 555 CD11a antibody CL555-65177 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |