CD109 Monoclonal antibody
CD109 Monoclonal Antibody for FC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
FC, WB,ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2H8G7
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive FC detected in | Jurkat cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Flow Cytometry (FC) | FC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
60179-1-Ig targets CD109 in WB, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, WB,ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD109 molecule |
| Synonyms | CD109, CD109 antigen, CD109 molecule, CPAMD7, p180, Platelet specific Gov antigen, r150, RP11 525G3.1 |
| Calculated Molecular Weight | 162 kDa |
| Observed Molecular Weight | 162 kDa |
| GenBank Accession Number | BC152996 |
| Gene Symbol | CD109 |
| Gene ID (NCBI) | 135228 |
| RRID | AB_10892668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | Q6YHK3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily. It is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein of approximately 170kd which is found on the cell surface of platelets, activated T-cells, and endothelial cells. CD109 binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta). CD109 has been identified as part of the TGF-beta receptor system in human keratinocytes. Up regulation of CD109 expression has been observed in several different types of tumor.CD109 is a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the a2-macroglobulin/C3, C4, C5 family of thioester-containing proteins. There're 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. The antibody can recognize all these 4 isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD109 antibody 60179-1-Ig | Download protocol |
| FC protocol for CD109 antibody 60179-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Oral Implants Res Characterization of macrophages infiltrating peri-implantitis lesions. | ||
Drug Dev Res Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. | ||
Mol Carcinog Tandem mass tag (TMT) quantitative protein analysis-based proteomics and parallel reaction monitoring (PRM) validation revealed that MST4 accelerates osteosarcoma proliferation by increasing MRC2 activity |