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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of Jurkat using 65566-1-MR (same clone as 65566-1-PBS)

    FC experiment of Jurkat using 65566-1-MR (same clone as 65566-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recombinant Antibody (65566-1-MR, Clone: H4B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 65566-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 65566-1-MR (same clone as 65566-1-PBS)

    IF Staining of HeLa using 65566-1-MR (same clone as 65566-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD107b / LAMP2 antibody (65566-1-MR, Clone: H4B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 65566-1-PBS in a different storage buffer formulation.

Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recombinant Antibody, PBS Only

CD107b / LAMP2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. 65566-1-PBS

产品说明书

CloneNo. H4B4

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

CD107b, LAMP2, CD107 antigen-like family member B, H4B4, LAMP 2

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.

产品信息

65566-1-PBS targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

Human adherent peripheral blood cells

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 CD107b, LAMP2, CD107 antigen-like family member B, H4B4, LAMP 2
计算分子量 45 kDa
GenBank蛋白编号BC002965
基因名称 LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13473
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

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