验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-SF98266-2 targets CD107a / LAMP1 in IF/ICC, FC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg2611 Product name: Recombinant Human CD107a/LAMP1 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 26-381 aa of NM_005561.4 Sequence: ASAAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENS 种属同源性预测 |
| 宿主/类型 | Rabbit / scFv |
| 抗体类别 | Recombinant |
| 产品类型 | Single-chain variable fragment antibody (scFv) |
| 全称 | lysosomal-associated membrane protein 1 |
| 别名 | CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, LAMP 1 |
| 计算分子量 | 45 kDa |
| GenBank蛋白编号 | NM_005561.4 |
| 基因名称 | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| RRID | AB_3748010 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 来源 | Anti-Human CD107a / LAMP1 scFv from clone 242116E8, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus. |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-SF98266-2 | Download protocol |
| IF protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-SF98266-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
