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验证数据展示

  • FC experiment of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

    FC experiment of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a / LAMP1 (65051-1-Ig, Clone:H4A3) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

  • WB analysis using 65051-1-Ig (same clone as 65051-1-PBS)

    WB analysis using 65051-1-Ig (same clone as 65051-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 65051-1-Ig (CD107a / LAMP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

    IF Staining of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

Anti-Human CD107a / LAMP1 (H4A3), PBS Only

CD107a / LAMP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Cat No. 65051-1-PBS

产品说明书

CloneNo. H4A3

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

WB, IF/ICC, FC (Intra)

别名

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.

产品信息

65051-1-PBS targets CD107a / LAMP1 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Human adherent peripheral blood cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 lysosomal-associated membrane protein 1
别名 CD107a, LAMP1, LAMP-1, LAMP 1, H4A3
计算分子量45 kDa
观测分子量100-120 kDa
GenBank蛋白编号BC006345
基因名称 LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
RRIDAB_2881467
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP11279
储存缓冲液 PBS only , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).