验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, HeLa cells, K-562 cells, THP-1 cells |
Positive IHC detected in | human tonsillitis tissue, human kidney tissue, human lymphoma tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:225-1:900 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
66582-1-Ig targets CD100 in WB, IHC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | CD100 fusion protein Ag26461 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D |
别名 | A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D |
计算分子量 | 96 kDa |
观测分子量 | 150 kDa |
GenBank蛋白编号 | BC054500 |
基因名称 | SEMA4D/CD100 |
Gene ID (NCBI) | 10507 |
RRID | AB_2881942 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q92854 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.
实验方案
Product Specific Protocols | |
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WB protocol for CD100 antibody 66582-1-Ig | Download protocol |
IHC protocol for CD100 antibody 66582-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |