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验证数据展示

  • IF Staining of MCF-7 using CL488-67539

    IF Staining of MCF-7 using CL488-67539

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 CCT8 antibody (CL488-67539, Clone: 1A7C3 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-67539

    FC experiment of MCF-7 using CL488-67539

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CCT8 (CL488-67539, Clone:1A7C3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CCT8 Monoclonal antibody

CCT8 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67539

产品说明书

CloneNo. 1A7C3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

C21orf112, CCT theta, CCTQ, CCT-theta, Chaperonin containing T-complex polypeptide 1 subunit 8

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67539-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67539 targets CCT8 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30082

Product name: Recombinant human CCT8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 146-497 aa of BC012584

Sequence: FNVDNIRVCKILGSGISSSSVLHGMVFKKETEGDVTSVKDAKIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAIADTGANVVVTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPVLEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAVDDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIPRALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILDTYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQND

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 8 (theta)
别名 C21orf112, CCT theta, CCTQ, CCT-theta, Chaperonin containing T-complex polypeptide 1 subunit 8
计算分子量 548 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC012584
基因名称 CCT8
Gene ID (NCBI) 10694
RRIDAB_3084353
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP50990
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CCT8 antibody CL488-67539Download protocol
IF protocol for CL Plus 488 CCT8 antibody CL488-67539Download protocol
Standard Protocols
Click here to view our Standard Protocols
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