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验证数据展示

  • WB analysis of mouse testis using 24896-1-AP (same clone as 24896-1-PBS)

    WB analysis of mouse testis using 24896-1-AP (same clone as 24896-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 24896-1-AP (same clone as 24896-1-PBS)

    IHC staining of human stomach using 24896-1-AP (same clone as 24896-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24896-1-AP (CCT2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCT2 antibody included
  • IF Staining of NIH/3T3 using 24896-1-AP (same clone as 24896-1-PBS)

    IF Staining of NIH/3T3 using 24896-1-AP (same clone as 24896-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CCT2 antibody (24896-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CCT2 Polyclonal antibody, PBS Only

CCT2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24896-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CCT2 Ready-To-Use IHC Kit. CCT2 primary antibody included.

产品信息

24896-1-PBS targets CCT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19264

Product name: Recombinant human CCT2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 186-535 aa of BC113516

Sequence: AVLRLKGSGNLEAIHIIKKLGGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 2 (beta)
别名 PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta
计算分子量 535 aa, 57 kDa
观测分子量 50-58 kDa
GenBank蛋白编号BC113516
基因名称 CCT2
Gene ID (NCBI) 10576
RRIDAB_2879783
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78371
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)

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