验证数据展示
经过测试的应用
| Positive WB detected in | T-47D cells, MCF-7 cells, K-562 cells, C6 cells, mouse spleen tissue, rat spleen tissue |
| Positive IF/ICC detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
86180-5-RR targets CCR7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Eg2354 Product name: recombinant human CCR7 protein Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 25-59 aa of BC035343 Sequence: QDEVTDDYIGDNTTVDYTLFESLCSKKDVRNFKAW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) receptor 7 |
| 别名 | BLR2, C C chemokine receptor type 7, C C CKR 7, C-C chemokine receptor type 7, CC CKR 7 |
| 计算分子量 | 378 aa, 43 kDa |
| 观测分子量 | 40-43 kDa |
| GenBank蛋白编号 | BC035343 |
| 基因名称 | CCR7 |
| Gene ID (NCBI) | 1236 |
| RRID | AB_3744690 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P32248 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C-C chemokine receptor type 7 (CCR7) , which belongs to the G-protein coupled receptor 1 family, is also a receptor for the MIP-3-beta chemokine, and probablely acts as mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. CCR7 is specially expressed in various lymphoid tissues and activated B- and T-lymphocytes, and can be strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CCR7 antibody 86180-5-RR | Download protocol |
| WB protocol for CCR7 antibody 86180-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Three-Dimensional Bioprinted Scaffolds Loaded with Multifunctional Magnesium-Based Metal-Organic Frameworks Improve the Senescence Microenvironment Prompting Aged Bone Defect Repair. | ||
Commun Biol CCR7-PERK-CREB3L1 signaling mediates tumor-associated macrophages-derived SPP1 promotion of oral squamous cell carcinoma. |
