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验证数据展示

  • WB analysis using 86180-5-RR (same clone as 86180-5-PBS)

    WB analysis using 86180-5-RR (same clone as 86180-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86180-5-RR (CCR7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86180-5-PBS in a different storage buffer formulation.

  • WB analysis using 86180-5-RR (same clone as 86180-5-PBS)

    WB analysis using 86180-5-RR (same clone as 86180-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86180-5-RR (CCR7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86180-5-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 86180-5-RR (same clone as 86180-5-PBS)

    IF Staining of K-562 using 86180-5-RR (same clone as 86180-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CCR7 antibody (86180-5-RR, Clone: 251886C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86180-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86180-5-RR

    Affinity and Kinetic Characterization of 86180-5-RR

    Biolayer interferometry (BLl) kinetic assays of 86180-5-RR against Human CCR7 were performed. The affinity constant is 8.06 nM.

CCR7 Recombinant monoclonal antibody, PBS Only

CCR7 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86180-5-PBS

产品说明书

CloneNo. 251886C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

BLR2, C C chemokine receptor type 7, C C CKR 7, C-C chemokine receptor type 7, CC CKR 7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86180-5-PBS targets CCR7 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg2354

Product name: recombinant human CCR7 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 25-59 aa of BC035343

Sequence: QDEVTDDYIGDNTTVDYTLFESLCSKKDVRNFKAW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) receptor 7
别名 BLR2, C C chemokine receptor type 7, C C CKR 7, C-C chemokine receptor type 7, CC CKR 7
计算分子量 378 aa, 43 kDa
观测分子量40-43 kDa
GenBank蛋白编号BC035343
基因名称 CCR7
Gene ID (NCBI) 1236
RRIDAB_3744690
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP32248
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C-C chemokine receptor type 7 (CCR7) , which belongs to the G-protein coupled receptor 1 family, is also a receptor for the MIP-3-beta chemokine, and probablely acts as mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. CCR7 is specially expressed in various lymphoid tissues and activated B- and T-lymphocytes, and can be strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

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