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CCR6 Monoclonal antibody

CCR6 Monoclonal Antibody for FC, IF/ICC,IF-P, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

FC, IF/ICC,IF-P, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C11A9

Cat No : 66801-1-Ig

Print datasheet

Synonyms

BN 1, C C chemokine receptor type 6, C C CKR 6, CC CKR 6, CCR 6, CCR6, CD196, Chemokine receptor like 3, CKR L3, CKR6, CKRL3, CMKBR6, DCR2, DRY 6, DRY6, G protein coupled receptor 29, GPR CY4, GPR29, GPRCY4, LARC receptor, STRL22

验证数据展示

  • IF Staining of Jurkat using 66801-1-Ig

    IF Staining of Jurkat using 66801-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of Jurkat using 66801-1-Ig

    FC experiment of Jurkat using 66801-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CCR6 (66801-1-Ig, Clone:1C11A9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human tonsillitis using 66801-1-Ig

    IHC staining of human tonsillitis using 66801-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66801-1-Ig

    IHC staining of human tonsillitis using 66801-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 66801-1-Ig

    IF Staining of human tonsillitis using 66801-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells, human tonsillitis tissue
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

66801-1-Ig targets CCR6 in IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF/ICC,IF-P, IHC, ELISA
Cited ApplicationsIHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CCR6 fusion protein Ag24195 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name chemokine (C-C motif) receptor 6
Synonyms BN 1, C C chemokine receptor type 6, C C CKR 6, CC CKR 6, CCR 6, CCR6, CD196, Chemokine receptor like 3, CKR L3, CKR6, CKRL3, CMKBR6, DCR2, DRY 6, DRY6, G protein coupled receptor 29, GPR CY4, GPR29, GPRCY4, LARC receptor, STRL22
Calculated Molecular Weight 42 kDa
GenBank Accession NumberBC037960
Gene Symbol CCR6
Gene ID (NCBI) 1235
RRIDAB_2882144
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51684
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCR6, also known as CD196, is a C-C chemokine receptor that belongs to family A of G protein-coupled receptor superfamily. It is expressed in most B cells, memory T cells, and some subsets of dendritic cells (PMID: 17057190). CCR6 is a receptor for the C-C type chemokine CCL20 (PMID: 9169459). The ligand-receptor pair CCL20-CCR6 is responsible for the chemoattraction of immature dendritic cells, effector/memory T-cells, and B-cells and plays a role in skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and rheumatoid arthritis (PMID: 12948524). CCR6 polymorphism has been associated with rheumatoid arthritis susceptibility and CCR6 is involved in IL-17-driven autoimmunity in human diseases (PMID: 20453841).

实验方案

Product Specific Protocols
IHC protocol for CCR6 antibody 66801-1-IgDownload protocol
IF protocol for CCR6 antibody 66801-1-IgDownload protocol
FC protocol for CCR6 antibody 66801-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Neoplasia

Gut microbiota-derived short-chain fatty acids promote prostate cancer progression via inducing cancer cell autophagy and M2 macrophage polarization

Authors - Yufei Liu
View Article