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验证数据展示

  • WB analysis using 30420-1-AP

    WB analysis using 30420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30420-1-AP (CCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 30420-1-AP

    IF Staining of THP-1 using 30420-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CCR2 antibody (30420-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

CCR2 Polyclonal antibody

CCR2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30420-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CC-CKR-2, C-C CKR-2, CC CKR 2, C-C chemokine receptor type 2, C C CKR 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHaCaT cells, K-562 cells, THP-1 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30420-1-AP targets CCR2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CCR2 fusion protein Ag33071 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) receptor 2
别名 CC-CKR-2, C-C CKR-2, CC CKR 2, C-C chemokine receptor type 2, C C CKR 2
计算分子量 374 aa, 42 kDa
观测分子量70 kDa
GenBank蛋白编号BC074751
基因名称 CCR2
Gene ID (NCBI) 729230
RRIDAB_3669721
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41597
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCR2 (C-C motif chemokine receptor-2), is a 42-kDa transmembrane protein highly expressed in bone marrow and lymph nodes (PMID: 16150057). It belongs to the G-protein-coupled seven-transmembrane receptor superfamily. CCR2 is the key functional receptor for MCP-1 (Monocyte chemoattractant protein-1) (PMID: 8146186). CCR2 and monocyte chemoattractant proteins (MCPs) play pivotal roles in the development of inflammatory responses and are crucial for the recruitment of immune cells to sites of inflammation. (PMID: 19441905). This antibody recognizes a homodimer consisting of its isoform1 and isoform2 (70 kDa).

实验方案

Product Specific Protocols
WB protocol for CCR2 antibody 30420-1-APDownload protocol
IF protocol for CCR2 antibody 30420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols