验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22071-1-AP targets CCR10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17212 Product name: Recombinant human CCR10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-82 aa of BC099840 Sequence: MGTEATEQVSWGHYSGDEEDAYSAEPLPELCYKADVQAFSRAFQPSVSLTVAALGLAGNGLVLATHLAARRAARSPTSAHLL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) receptor 10 |
| 别名 | CC-CKR-10, C-C CKR-10, CC CKR 10, C-C chemokine receptor type 10, C C CKR 10 |
| 计算分子量 | 362 aa, 38 kDa |
| 观测分子量 | 38-42 kDa |
| GenBank蛋白编号 | BC099840 |
| 基因名称 | CCR10 |
| Gene ID (NCBI) | 2826 |
| RRID | AB_11182921 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46092 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCR10 (also known as GPR2) is a G-protein coupled receptor for chemokines CCL27 (SCYA27) and CCL28 (SCYA28). CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (PMID: 11821900). CCR10-CCL28 interactions are thought to contribute to the accumulation of IgA Ab-secreting cells (ASC) on mucosal surfaces.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCR10 antibody 22071-1-AP | Download protocol |
| WB protocol for CCR10 antibody 22071-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression | ||
Front Immunol Single-Cell Sequencing Reveals the Transcriptome and TCR Characteristics of pTregs and in vitro Expanded iTregs. | ||
Mol Med Rep Knockdown of CCL28 inhibits endometriosis stromal cell proliferation and invasion via ERK signaling pathway inactivation | ||
Inflamm Regen Th22 is the effector cell of thymosin β15-induced hair regeneration in mice | ||
