CCR10 Polyclonal antibody
CCR10 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22071-1-AP targets CCR10 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB, FC, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CCR10 fusion protein Ag17212 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chemokine (C-C motif) receptor 10 |
| Synonyms | C C chemokine receptor type 10, C C CKR 10, CC CKR 10, CCR 10, CCR10, G protein coupled receptor 2, GPR2 |
| Calculated Molecular Weight | 362 aa, 38 kDa |
| Observed Molecular Weight | 38-42 kDa |
| GenBank Accession Number | BC099840 |
| Gene Symbol | CCR10 |
| Gene ID (NCBI) | 2826 |
| RRID | AB_11182921 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46092 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCR10 (also known as GPR2) is a G-protein coupled receptor for chemokines CCL27 (SCYA27) and CCL28 (SCYA28). CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (PMID: 11821900). CCR10-CCL28 interactions are thought to contribute to the accumulation of IgA Ab-secreting cells (ASC) to mucosal surfaces.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCR10 antibody 22071-1-AP | Download protocol |
| IHC protocol for CCR10 antibody 22071-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression | ||
Front Immunol Single-Cell Sequencing Reveals the Transcriptome and TCR Characteristics of pTregs and in vitro Expanded iTregs. | ||
Mol Med Rep Knockdown of CCL28 inhibits endometriosis stromal cell proliferation and invasion via ERK signaling pathway inactivation | ||
Inflamm Regen Th22 is the effector cell of thymosin β15-induced hair regeneration in mice |
