验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, PC-3 cells, MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13138-1-AP targets CCNL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CCNL1 fusion protein Ag3731 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cyclin L1 |
别名 | ania 6a, BM 001, CCNL1, Cyclin L, cyclin L1, PRO1073, UNQ530/PRO1073 |
计算分子量 | 526 aa, 60 kDa |
观测分子量 | 46 kDa |
GenBank蛋白编号 | BC007081 |
基因名称 | CCNL1 |
Gene ID (NCBI) | 57018 |
RRID | AB_10640299 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UK58 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCNL1, also named as Cyclin-L1, contains a RS region and belongs to the cyclin family. CCNL1 localizes in the nucleus speckle and is Overexpressed in primary tumors of head and neck squamous cell carcinomas (HNSCC). CCNL1 as transcriptional regulator participates in regulating the pre-mRNA splicing process. It is seems to be involved in the regulation of RNA polymerase II (pol II). CCNL1 functions in association with cyclin-dependent kinases (CDKs) and has a role in the second step of splicing. It may be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC). The calculated molecular weight of CCNL1 is about 55-60 kDa, but we detected a 40-50 kDa isoform by western blotting.
实验方案
Product Specific Protocols | |
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WB protocol for CCNL1 antibody 13138-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |