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cyclin I Polyclonal antibody

cyclin I Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16357-1-AP

Print datasheet

Synonyms

CCNI, CYC1, cyclin I, CYI

验证数据展示

  • WB analysis using 16357-1-AP

    WB analysis using 16357-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16357-1-AP (cyclin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 16357-1-AP

    IHC staining of human heart using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16357-1-AP

    IHC staining of human heart using 16357-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16357-1-AP (cyclin I antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, human brain tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16357-1-AP targets cyclin I in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen cyclin I fusion protein Ag0493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin I
Synonyms CCNI, CYC1, cyclin I, CYI
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC004975
Gene Symbol CCNI
Gene ID (NCBI) 10983
RRIDAB_2275606
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14094
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for cyclin I antibody 16357-1-APDownload protocol
IHC protocol for cyclin I antibody 16357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols