×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 85895-1-RR (same clone as 85895-1-PBS)

    WB analysis using 85895-1-RR (same clone as 85895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85895-1-RR (Cyclin B1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85895-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02202-2

    Sandwich ELISA sample test of MP02202-2

    The mean Cyclin B1 concentration was determined to be 21.36 ng/mL in K-562 cell extract based on a 2.50 mg/mL extract load and 9.28 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02202-2

    Sandwich ELISA standard curve of MP02202-2

    Sandwich ELISA standard curve of MP02202-2, Human Cyclin B1 Recombinant Matched Antibody Pair - PBS only. 85895-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29426. 85895-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Cytometric bead array sample test of MP02202-1

    Cytometric bead array sample test of MP02202-1

    The mean Cyclin B1 concentration was determined to be 33.7 ng/mL in Hela cell extract based on a 1.3 mg/mL extract load.

  • Cytometric bead array standard curve of MP02202-1

    Cytometric bead array standard curve of MP02202-1

    Cytometric bead array standard curve of MP02202-1, Cyclin B1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85895-2-PBS. Detection antibody: 85895-1-PBS. Standard: Ag29426. Range: 1.563-100 ng/mL.

  • Affinity of 85895-1-RR

    Affinity of 85895-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85895-1-RR against Human Cyclin B1 were performed. The affinity constant is 3.29 nM.

Cyclin B1 Recombinant monoclonal antibody, PBS Only (Detector)

Cyclin B1 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 85895-1-PBS

产品说明书

CloneNo. 250227H1

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CCNB1 Ready-To-Use IHC Kit. CCNB1 primary antibody included.

产品信息

85895-1-PBS targets Cyclin B1 as part of a matched antibody pair:

MP02202-1: 85895-2-PBS capture and 85895-1-PBS detection (validated in Cytometric bead array)

MP02202-2: 85895-4-PBS capture and 85895-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag29426

Product name: Recombinant human CCNB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-140 aa of BC006510

Sequence: MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin B1
别名 CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1
计算分子量 48 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC006510
基因名称 Cyclin B1
Gene ID (NCBI) 891
RRIDAB_3744453
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14635
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

||
New chat

Able™

正在加载，请稍候...