验证数据展示
经过测试的应用
| Positive WB detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
| Positive IF/ICC detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87376-1-RR targets CCL4L1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg5335 Product name: Recombinant Human CCL4L1 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 24-92 aa of N/A Sequence: APMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRGKQVCADPSESWVQEYVYDLELN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 4-like 1 |
| 别名 | ACT-2, C-C motif chemokine 4, CCL4, G-26 T-lymphocyte-secreted protein, HC21 |
| 计算分子量 | 10 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | N/A |
| 基因名称 | CCL4L1 |
| Gene ID (NCBI) | 9560 |
| RRID | AB_3745495 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8NHW4-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCL4L1 encodes a secreted chemokine ligand in the intercrine beta family that functions in inflammatory and immunoregulatory processes. The encoded 92-amino-acid protein is similar to chemokine (C-C motif) ligand 4, and the gene belongs to a cluster of cytokine genes on the q-arm of chromosome 17. CCL4L1 is localized to the extracellular region, placing it in a position to act in intercellular signaling.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CCL4L1 antibody 87376-1-RR | Download protocol |
| WB protocol for CCL4L1 antibody 87376-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
