验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | human monocyte-derived mature dendritic cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-FcA98280 targets CCL22/MDC in FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg1974 Product name: Recombinant Human CCL22 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 25-93 aa of BC027952 Sequence: GPYGANMEDSVCCRDYVRYRLPLRVVKHFYWTSDSCPRPGVVLLTFRDKEICADPRVPWVKMILNKLSQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 22 |
| 别名 | CCL22, MDC, C C motif chemokine 22, CC chemokine STCP-1, C-C motif chemokine 22 |
| 计算分子量 | 93 aa, 11 kDa |
| GenBank蛋白编号 | BC027952 |
| 基因名称 | CCL22/MDC |
| Gene ID (NCBI) | 6367 |
| 偶联类型 | Biotin |
| 最大激发/发射波长 | - |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O00626 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CCL22, also known as macrophage-derived chemokine (MDC), is a member of the CC chemokine family. It is primarily produced by myeloid cells such as macrophages and dendritic cells (DCs) under steady-state conditions. CCL22 plays a significant role in immune responses by regulating the migration of different subsets of leukocytes. It shares the same receptor, CCR4, which is predominantly expressed by Th2 cells, making CCL22 a key mediator in the development of Th2-dominant diseases such as atopic dermatitis and asthma. Elevated serum levels of CCL22 are observed in patients with these conditions. CCL22 is also expressed in several types of tumor cells and Foxp3+ regulatory T cells (Tregs), suggesting its involvement in attracting Tregs into the tumor microenvironment to evade immune attacks.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin CCL22/MDC antibody Biotin-FcA98280 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
