×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 85861-1-RR

    WB analysis using 85861-1-RR

    Mouse CCL19 Recombinant protein were subjected to SDS PAGE followed by western blot with 85861-1-RR (Ccl19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85861-1-RR

    WB analysis using 85861-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85861-1-RR (Ccl19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 85861-1-RR

    IHC staining of mouse spleen using 85861-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85861-1-RR (CCL19/MIP-3 beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 85861-1-RR

    IHC staining of mouse spleen using 85861-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85861-1-RR (CCL19/MIP-3 beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCL19/MIP-3 beta Recombinant monoclonal antibody

CCL19/MIP-3 beta Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85861-1-RR

产品说明书

CloneNo. 242867C1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

C-C motif chemokine 19, Ccl19, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inRecombinant protein, Transfected with Mouse Ccl19 Transfected HEK-293T cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85861-1-RR targets CCL19/MIP-3 beta in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg3124

Product name: Recombinant Mouse CCL19/MIP-3 beta protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 26-108 aa of NM_011888.2

Sequence: GANDAEDCCLSVTQRPIPGNIVKAFRYLLNEDGCRVPAVVFTTLRGYQLCAPPDQPWVDRIIRRLKKSSAKNKGNSTRRSPVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 19
别名 C-C motif chemokine 19, Ccl19, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine
计算分子量12 kDa
GenBank蛋白编号NM_011888.2
基因名称 Ccl19
Gene ID (NCBI) 24047
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO70460
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

实验方案

Product Specific Protocols
IHC protocol for CCL19/MIP-3 beta antibody 85861-1-RRDownload protocol
WB protocol for CCL19/MIP-3 beta antibody 85861-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...