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验证数据展示

  • FC experiment of Transfected HEK-293T using 98542-1-RR

    FC experiment of Transfected HEK-293T using 98542-1-RR

    1x10^6 HEK-293T cells and CCL12/MCP-5-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse Ccl12 Rabbit RecAb (98542-1-RR, Clone:250386G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of Transfected HEK-293T using 98542-1-RR

    FC experiment of Transfected HEK-293T using 98542-1-RR

    1x10^6 HEK-293T cells (left) or CCL12/MCP-5-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL12/MCP-5 Rabbit RecAb (98542-1-RR, Clone:250386G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 98542-1-RR

    Affinity and Kinetic Characterization of 98542-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98542-1-RR against Mouse CCL12/MCP-5 were performed. The affinity constant is 94.9 pM.

Anti-Mouse CCL12/MCP-5 Rabbit Recombinant Antibody

CCL12/MCP-5 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98542-1-RR

产品说明书

CloneNo. 250386G10

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

C-C motif chemokine 12, Ccl12, MCP-1-related chemokine, Mcp5, MCP-5

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98542-1-RR targets CCL12/MCP-5 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原

CatNo: Eg3315

Product name: Recombinant Mouse CCL12/MCP-5 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 23-104 aa of NM_011331.2

Sequence: GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 12
别名 C-C motif chemokine 12, Ccl12, MCP-1-related chemokine, Mcp5, MCP-5
计算分子量12KD
GenBank蛋白编号NM_011331.2
基因名称 Ccl12
Gene ID (NCBI) 20293
RRIDAB_3746167
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ62401
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils.

实验方案

Product Specific Protocols
FC protocol for CCL12/MCP-5 antibody 98542-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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