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验证数据展示

  • WB analysis using 24689-1-AP

    WB analysis using 24689-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24689-1-AP (CCDC99 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24689-1-AP

    WB analysis of HeLa using 24689-1-AP

    WB result of CCDC99 antibody (24689-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC99 transfected HeLa cells.

  • IHC staining of human liver cancer using 24689-1-AP

    IHC staining of human liver cancer using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCDC99 antibody included
  • IHC staining of human liver cancer using 24689-1-AP

    IHC staining of human liver cancer using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCDC99 antibody included
  • IP experiment of HEK-293 using 24689-1-AP

    IP experiment of HEK-293 using 24689-1-AP

    IP result of anti-CCDC99 (IP:24689-1-AP, 4ug; Detection:24689-1-AP 1:2000) with HEK-293 cells lysate 1400 ug.

  • IHC staining of human ovary cancer using 24689-1-AP

    IHC staining of human ovary cancer using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCDC99 antibody included
  • IHC staining of rat brain using 24689-1-AP

    IHC staining of rat brain using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCDC99 antibody included
  • IHC staining of rat ovary using 24689-1-AP

    IHC staining of rat ovary using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCDC99 antibody included
  • IHC staining of rat testis using 24689-1-AP

    IHC staining of rat testis using 24689-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24689-1-AP (CCDC99 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCDC99 antibody included
  • Featured Product
  • KD/KO Validated

CCDC99 Polyclonal antibody

CCDC99 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 24689-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

别名

Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99, hSpindly, MU-RMS-40.4A, Rhabdomyosarcoma antigen MU-RMS-40.4A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HEK293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human ovary cancer tissue, rat testis tissue, mouse testis tissue, rat ovary tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CCDC99 Ready-To-Use IHC Kit. CCDC99 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24689-1-AP targets CCDC99 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag20430

Product name: Recombinant human CCDC99 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 259-605 aa of BC012568

Sequence: AEVEDRRAAMERQLISMKVKYQSLKKQNVFNREQMQRMKLQIATLLQMKGSQTEFEQQERLLAMLEQKNGEIKHLLGEIRNLEKFKNLYDSMESKPSVDSGTLEDNTYYTDLLQMKLDNLNKEIESTKGELSIQRMKALFESQRALDIERKLFANERCLQLSESENMKLRAKLDELKLKYEPEETVEVPVLKKRREVLPVDITTAKDACVNNSALGGEVYRLPPQKEETQSCPNSLEDNNLQLEKSVSIHTPVVSLSPHKNLPVDMQLKKEKKCVKLIGVPADAEALSERSGNTPNSPRLAAESKLQTEVKEGKETSSKLEKETCKKSHPILYVSSKSTPETQCPQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 99
别名 Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99, hSpindly, MU-RMS-40.4A, Rhabdomyosarcoma antigen MU-RMS-40.4A
计算分子量 605 aa, 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC012568
基因名称 CCDC99
Gene ID (NCBI) 54908
RRIDAB_2879673
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EA4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CCDC99 antibody 24689-1-APDownload protocol
IP protocol for CCDC99 antibody 24689-1-APDownload protocol
WB protocol for CCDC99 antibody 24689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cells

Identification of Ku70 Domain-Specific Interactors Using BioID2.

Authors - Sanna Abbasi
View Article

Mol Biol Cell

Dynamic Phosphorylation of MIS12 Ensures Accurate Kinetochore-Microtubule Attachment by Expanding the Fibrous Corona

Authors - Qi Li
View Article
humanWB

PLoS One

Inhibiting the expression of spindle appendix cooled coil protein 1 can suppress tumor cell growth and metastasis and is associated with cancer immune cells in esophageal squamous cell carcinoma

Authors - Tao Liu
  • KD Validated
View Article
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