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CCDC88C Polyclonal antibody

CCDC88C Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25769-1-AP

Print datasheet

Synonyms

CCDC88C, DAPLE, hDaple, HkRP2, Hook related protein 2, KIAA1509, Protein Daple

验证数据展示

  • WB analysis of HEK-293 using 25769-1-AP

    WB analysis of HEK-293 using 25769-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25769-1-AP (CCDC88C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25769-1-AP

    IHC staining of human testis using 25769-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25769-1-AP (CCDC88C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 25769-1-AP

    IHC staining of human testis using 25769-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25769-1-AP (CCDC88C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 25769-1-AP

    IF Staining of HEK-293 using 25769-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 25769-1-AP (CCDC88C antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 25769-1-AP is CCDC88C Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivitymouse
Immunogen CCDC88C fusion protein Ag22711 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 88C
Synonyms CCDC88C, DAPLE, hDaple, HkRP2, Hook related protein 2, KIAA1509, Protein Daple
Calculated Molecular Weight 2028 aa, 228 kDa
Observed Molecular Weight 250 kDa, 100 kDa
GenBank Accession NumberBC028565
Gene Symbol CCDC88C
Gene ID (NCBI) 440193
RRIDAB_2880233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P219
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC88C antibody 25769-1-APDownload protocol
IHC protocol for CCDC88C antibody 25769-1-APDownload protocol
IF protocol for CCDC88C antibody 25769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.

Authors - Katie S Kindt
View Article
mouse

Sci Adv

RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells

Authors - Anil Akturk
View Article