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验证数据展示

  • WB analysis using 31484-1-AP (same clone as 31484-1-PBS)

    WB analysis using 31484-1-AP (same clone as 31484-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31484-1-AP (CCDC76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 31484-1-AP (same clone as 31484-1-PBS)

    IHC staining of human placenta using 31484-1-AP (same clone as 31484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 31484-1-AP (same clone as 31484-1-PBS)

    IHC staining of mouse testis using 31484-1-AP (same clone as 31484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 31484-1-AP (same clone as 31484-1-PBS)

    IF Staining of HeLa using 31484-1-AP (same clone as 31484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC76 antibody (31484-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

CCDC76 Polyclonal antibody, PBS Only

CCDC76 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31484-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

tRNA [Gm4] methyltransferase, TRMT13

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31484-1-PBS targets CCDC76 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34110

Product name: Recombinant human CCDC76 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 132-481 aa of BC075811

Sequence: GLNSTLKDHIMSHPALHDALNDPKNGDSATKHLKQQASILGNIENLKLLGPRRCFVEFGAGKGKLSHWVDIALKDAEKVHFILVEKVTTRFKVDGKHRKKNSVFERLQIDIQHLCLNKIPVLREEKLPVVGIGKHLCGMATDLALRCLVETYAASFEERNEEPLAKRIKNDKTEKEIYTLAKEGNEKNVPEKWNPVAGIVIALCCHHRCDWRHYVGKEYFRALGLGAVEFHYFQRMSSWATCGMRKTSLETSNSTTKRQDNQNDDSEEHDDGGYRITDDGADCLPGLLSVEEKKKIGHLCKLLIDQGRIQYLQQKGFSPALQYYTDPLVSLENVLLTALPNHSSSPETTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 76
别名 tRNA [Gm4] methyltransferase, TRMT13
观测分子量54 kDa
GenBank蛋白编号BC075811
基因名称 CCDC76
Gene ID (NCBI) 54482
RRIDAB_3670001
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NUP7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCDC76 (coiled‐coil domain‐containing protein 76), also known as the human tRNA Nm4 methyltransferase (hTrmt13) is an uncharacterized human protein containing 481 amino acid residues that shares 27% sequence similarity with yeast Trm13p. The expression of CCDC76 was significantly associated with patient survival in breast carcinoma and liver and renal cancer patients (PMID: 34850409).

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