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验证数据展示

  • WB analysis using 31484-1-AP

    WB analysis using 31484-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31484-1-AP (CCDC76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 31484-1-AP

    IHC staining of human placenta using 31484-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 31484-1-AP

    IHC staining of mouse testis using 31484-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 31484-1-AP

    IF Staining of HeLa using 31484-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC76 antibody (31484-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

CCDC76 Polyclonal antibody

CCDC76 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31484-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

tRNA [Gm4] methyltransferase, TRMT13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, LNCaP cells
Positive IHC detected inhuman placenta tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31484-1-AP targets CCDC76 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CCDC76 fusion protein Ag34110 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 76
别名 tRNA [Gm4] methyltransferase, TRMT13
观测分子量54 kDa
GenBank蛋白编号BC075811
基因名称 CCDC76
Gene ID (NCBI) 54482
RRIDAB_3670001
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NUP7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC76 (coiled‐coil domain‐containing protein 76), also known as the human tRNA Nm4 methyltransferase (hTrmt13) is an uncharacterized human protein containing 481 amino acid residues that shares 27% sequence similarity with yeast Trm13p. The expression of CCDC76 was significantly associated with patient survival in breast carcinoma and liver and renal cancer patients (PMID: 34850409).

实验方案

Product Specific Protocols
WB protocol for CCDC76 antibody 31484-1-APDownload protocol
IHC protocol for CCDC76 antibody 31484-1-APDownload protocol
IF protocol for CCDC76 antibody 31484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols