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CWC25 Polyclonal antibody

CWC25 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24416-1-AP

Print datasheet

Synonyms

CCDC49, CWC25

验证数据展示

  • WB analysis of HeLa using 24416-1-AP

    WB analysis of HeLa using 24416-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24416-1-AP (CWC25 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24416-1-AP

    IHC staining of human testis using 24416-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24416-1-AP (CWC25 antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 24416-1-AP

    IF Staining of HepG2 using 24416-1-AP

    Immunofluorescent analysis of HepG2 cells using 24416-1-AP (CWC25 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24416-1-AP is CWC25 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen CWC25 fusion protein Ag17037 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 49
Synonyms CCDC49, CWC25
Calculated Molecular Weight 425 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC008833
Gene Symbol CCDC49
Gene ID (NCBI) 54883
RRIDAB_2879535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXE8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CWC25 antibody 24416-1-APDownload protocol
IHC protocol for CWC25 antibody 24416-1-APDownload protocol
IF protocol for CWC25 antibody 24416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols