验证数据展示
经过测试的应用
| Positive WB detected in | MOLT-4 cells, Jurkat cells, K-562 cells, HL-60 cells |
| Positive IF/ICC detected in | U2OS cells |
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67755-1-Ig targets CCDC45 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag27836 Product name: Recombinant human CCDC45 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 86-349 aa of BC009518 Sequence: GENIVKGDKESIKNLLEIFDGLLEYLTERISETSHEKSETEQYFKESDRGERLEEPESTKESKSSWKRVSFGRCSLSSEMLGPSWDGDEAESTGEIIRLGDTAHTFSLRSNGAQCPNEMLSKKALASPSSKSHEDMLYPPSVLSKSRTSFVEDTETLSVSGIPNARKLGEPIRAAIPLHPPYHPSEPRAPCPIGKEYLHSSHCSPAVNSTGEHTEFSGDLDDGLFLISKLPKGSKWEVYPAQVQGPRTRKPPKGKRNENRATAS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 45 |
| 别名 | CCDC45, DKFZp667E1824 |
| 计算分子量 | 821 aa, 95 kDa |
| 观测分子量 | 95-100 kDa |
| GenBank蛋白编号 | BC009518 |
| 基因名称 | CCDC45 |
| Gene ID (NCBI) | 90799 |
| RRID | AB_2918523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96GE4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CCDC45 antibody 67755-1-Ig | Download protocol |
| WB protocol for CCDC45 antibody 67755-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
